A dilution is a reduction in the concentration of a solution. A serial dilution is a series of repeated dilutions that provides a geometric dilution of the original solution. This is commonly performed in experiments that involve concentration curves on a logarithmic scale. Serial dilutions are used extensively in biochemistry and microbiology.
Items you will need
- Three or more test tubes
Fill test tube A with 10 mL of the solution and fill test tube B with 9 mL of a buffer to dilute the original solution. The buffer is typically water, but it also can be other liquids, depending on the solution in test tube A.
Draw 1 mL of the solution in test tube A with the pipette, transfer it to test tube B and mix the contents of test tube B. The solution in test B is 10 times more dilute than the solution in test tube A.
Fill test tube C with 9 mL of buffer. Transfer 1 mL of the solution in test tube B to test tube C and mix thoroughly. The solution in test tube C is 10 times more dilute than the solution in test tube B.
Examine the effects of serial dilution. The solution in test tube C is 10 times more dilute that the solution in test tube B, which is 10 times more dilute than the solution in test tube A. The solution in test tube C is, therefore, 10 x 10 = 100 times more dilute than the solution in test tube A.
Calculate the total dilution in a serial dilution. We can generalize the results of Step 4 by saying that the total dilution ratio Dt is given by the equation Dt = D1 x D2 x D3 x ... x Dn, where Di is the dilution ratio of the ith dilution.
Style Your World With Color
Let your clothes speak for themselves with this powerhouse hue.View Article
Explore a range of beautiful hues with the year’s must-have colors.View Article
Understand how color and its visual effects can be applied to your closet.View Article
Let your imagination run wild with these easy-to-pair colors.View Article