A dilution is a reduction in the concentration of a solution. A serial dilution is a series of repeated dilutions that provides a geometric dilution of the original solution. This is commonly performed in experiments that involve concentration curves on a logarithmic scale. Serial dilutions are used extensively in biochemistry and microbiology.
Items you will need
- Three or more test tubes
Fill test tube A with 10 mL of the solution and fill test tube B with 9 mL of a buffer to dilute the original solution. The buffer is typically water, but it also can be other liquids, depending on the solution in test tube A.
Draw 1 mL of the solution in test tube A with the pipette, transfer it to test tube B and mix the contents of test tube B. The solution in test B is 10 times more dilute than the solution in test tube A.
Fill test tube C with 9 mL of buffer. Transfer 1 mL of the solution in test tube B to test tube C and mix thoroughly. The solution in test tube C is 10 times more dilute than the solution in test tube B.
Examine the effects of serial dilution. The solution in test tube C is 10 times more dilute that the solution in test tube B, which is 10 times more dilute than the solution in test tube A. The solution in test tube C is, therefore, 10 x 10 = 100 times more dilute than the solution in test tube A.
Calculate the total dilution in a serial dilution. We can generalize the results of Step 4 by saying that the total dilution ratio Dt is given by the equation Dt = D1 x D2 x D3 x ... x Dn, where Di is the dilution ratio of the ith dilution.
Style Your World With Color
Create balance and growth throughout your wardrobe.View Article
Explore a range of cool greys with the year's top colors.View Article
Understand how color and its visual effects can be applied to your closet.View Article
See how the colors in your closet help determine your mood.View Article